Osmolarity: Concentration and Sucrose Solutions Essay

My chemical sort protrude and I conducted the test that estimates osmolarity by swap in encumbrance of stump spud genus Tubers, this was conducted in monastic order to explore the process of diffusion and osmosis and more importantly to investigate the psyche of Does contrary c at oncentrations of saccharose tooth roots arouse an effect on the last-place freight for the tater tubers? In this essay we estimated the osmolarity of potato tuber cores by submersing diametrical potato cores into saccharose effects of 0.0-0.6M, and weighing the potato. The results showed the freight unit of the potato tubers had the high upest pct change in weight meaning that they weighed more than the initial weight in sucrose resolvings from 0.0-0.3M it too showed that sucrose soaking ups from 0.4-0.6M the weight of the potato tubers decreased. My group and I concluded that the osmolarity of the potato was about 0.4M since the weight of the potato decreased by about -1.3%, which was the closest value to the initial weight of the potato tuber. We as well found that the potato was hypertonic to sucrose solutions of 0.0-0.3M and hypotonic to 0.5-0.6M.Introduction dispersion and osmosis be dickens types of passive express. Diffusion is a random consummation of molecules from an area of high preoccupation to an area of low tightfistedness. According to the keep back Biological Sciences, Osmosis is a type of diffusion that occurs when solutions are separated by a membrane that is permeably to some molecules but non to others, that is, a selectively permeable membrane (Scott 2011). To further explore the process of diffusion and osmosis, we conducted an examine that would demonstrate these processes and also investigate the question of do different concentrations of sucrose solutions have an effect on the final weight for the potato tubers? In my groups essay our goal was to estimate the osmolarity of potato tubers from weight change. The hypothesis fo r this experiment was, if the concentration of the sucrose solutions in which the potato cylinders are in is changed, so I contemplate that the final weight of the potato will also change.And the foretelling that my group and I formed was if the weight if the potato tuber changes when submerse in different sucrose concentrations, then I predict the weight change will decrease as the sucrose concentration increases. In my groups experiment, several potato tubers were tested in different sucrose solutions ranging from 0.0-0.6 M. The potato tubers were then subaqueous into tout ensemble the solutions to test osmolarity and to see what would happen to its mass if they were in different sucrose solutions. To fully understand the purpose and understand the results obtained there were trey major concepts important to know, they are hypertonic, hypotonic, and isotonic.According to the journal The American biota Teacher, An isotonic solution is when the solute concentration inside a s ystem is like to the solute concentration external of a system, thus resulting in no clear change of diffusion. In a hypertonic solution, the solute concentration outside of a system is larger than the solute concentration within a system, so piddle diffuses out of the system to attempt to even out the ratio inequality this results in the system shrinking in mass (Marvel, Kepler 2009). In a hypotonic solution however, the solute concentration is greater within the system than outside of the system, so water diffuses into the system this results in the system being bloated.Materials and MethodsThe materials that my group and I apply in our experiment was 1 large potato, a cork borer this is necessary to obtain seven potato tuber cylinders. Forceps were needed and a balance that weighs to the nearest 0.01g, a Petri traveling bag, razor blade, account towels, ruler, calculator, and also necessary for the experiment to work was sucrose solutions from 0.1-0.6 molar. Deionized wa ter was used to represent 0.0 molar in our experiment and seven 250ml plastic cups. outgrowth, my group and I obtained 50ml of deionized water and 100ml of each of the sucrose solutions and put each solution in separate and labeled 250ml paper cups. Then by employ a cork borer we obtained seven cylinders form the potato by pushing the borer by the length of the potato and removing the potato from the borer. making sure none of the cylinders were damaged, we modified the length of each cylinder to 5ml and recurrent this step seven sequences until we had a total of seven uninjured cylinders of equal length with the peels set asided from each length using a razor blade. We then place all seven cylinders into a Petri dish and kept them covered to prevent from drying out. Before weighing each of the cylinders we placed each one between folds of a napkin to blot out the sides and ends and then weighed them individually to the nearest hundredths of a gram on the balance. later on d oing this step we put down the results in our table.after weighing the potato cylinders we right off placed each in different molar solutions starting with 0.0M through 0.6M. subsequently the cylinders were submersed in the cups we recorded the time witch was 320 pm. We then took the cylinders out of their solutions at 430pm and calculated the brooding time to be 1hour 10 minutes. The instructions verbalize to leave for 1.5 hours to 2 hours but due to time constraints we took them out a little earlier. After removing the cylinders from each sample we blotted each with a paper towel to remove excess solution only.After doing this my group and I recorded the final weights of each of the cylinders in the chronological order in which they were initially placed, and recorded it in our table. After recording our data we finally calculated the percent weight change for each of the cylinders. Our group then decided what the proteans were and concur that the independent variable was th e concentration of the sucrose solution and the dependent variable was the percent change in weight. This experiment was repeated only once in the given time we had.ResultsThe osmotic concentration was determined by measuring the percent change in mass of the potato cylinders. interchange in mass was measured of seven solutions, each containing different levels of concentration 0, 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6M. The percent change in mass decreased as sucrose concentration increased, therefore, relative osmotic concentration also decreased as sucrose concentration increased. However, the osmotic concentration of 0.3 M sucrose solution was relatively greater than that of 0.2 M sucrose solution.In sucrose concentration 0.6 M, the osmotic concentration decreased almost double from that of 0.5, and significantly from those of all other sucrose concentrations. The osmotic concentrations were greater than secret code in sucrose solutions of 0, 0.1, 0.2, and 0.3 M these cells were hyp otonic, meaning the potato had more solute. The osmotic concentrations were less than zero in sucrose solutions of 0.4, 0.5, and 0.6 M these cells were hypertonic, meaning the solutions had less solute. Osmotic concentration decreased as sucrose concentration increased and cells became more voiceless. card 1 Data for Experiment Estimating Osmolarity by Change in encumbrance Sucrose Molarity (M)0.00.10.20.30.40.50.6Final weight (g)2.572.432.482.242.212.051.82Initial weight (g)2.232.182.282.032.242.192.06 tilt change (g)0.340.250.200.21-0.3-1.4-0.24% change in weight15.2%11.5%8.8%10.3%-1.3%-6.3%-11.7%DiscussionWhen starting this experiment my group and I formulated and agreed upon the hypothesis of if the concentration of the sucrose solutions in which the potato cylinders are in is changed, then I hypothesize that the final weight of the potato will also change. My group and I also agreed upon the prediction of if the weight of the potato tuber changes when submerged in different sucrose concentrations, then I predict the weight change will decrease as the sucrose concentration increases. After conducting the experiment and obtained our results, we found that our results support our hypothesis and prediction. The experiment back up our prediction because the sucrose solution diffused from areas of high concentration to areas of low concentration, thus asking the final weight of potato cylinders when submerged in variable amounts of sucrose concentrations. According to the article Diffusion, Osmosis and cellular phone Membranes,There are two ways that the molecules in a solution move passive transport and active transport. Active transport consumes that the cell use energy that it has obtained from feed to move the molecules (or larger particles) through the cell membrane. Passive transport does not require such energy expenditure, and occurs spontaneously (Mccandless 1998).Because the molecules in the sucrose solutions in our experiment were moving wit h the gradient meaning they were moving form areas of high concentration to areas of low concentration we found that the movement of the molecules was passive transport. The ruler means of passive transport is diffusion. Diffusion is the movement of molecules from a neighborhood in which they are highly concentrated to a region in which they are less concentrated. In the solutions ranging from 0.0-0.3M the potato acted as the system and the solution concentration inside the system was greater than outside which was the sucrose solution, so water diffused into the system (potato) and caused it to become bloated. In the sucrose solutions 0.4-0.6 it was hypertonic because the solution concentration was larger than the systems concentration so the cylinder decreased in size. This experiment allowed us to take a closer look at the biological process of action and how and wherefore it works the way it does.This experiment allowed us to a take a deeper look into the mechanisms of diffus ion and osmosis and apply it real life examples. According to the book, Cell and molecular biology concepts and experiments, When a diluted solution and a concentrated solution are separated by a membrane, there is a net transfer of the solvent from the diluted solution to the concentrated one. founding of water into root hairs and movement of water within the plant physical structure are good examples of osmosis (Karp 1991). Osmosis plays a significant role in life first, the entry of water in to the roots from the soil takes place by this process, cell to cell diffusion of water is controlled through this process, young cells require turgid condition for their growth which is fulfilled by osmosis, and last turgidness of cells is maintained by the process of osmosis (Karp 1999).A few errors were made in the experiment but none were significant enough to heavily affect our results. For example, the lengths of the individual potato cylinders may have differed slightly we may have made mistakes when measuring a specific amount of the sucrose concentrations. We also believe that the potato cylinders should have been incubated longer, ours incubated for 1 hour 10minutes and the instructions said to incubate for at least 1.5 hours.For the most part these mistakes seemed to be gloomy and not significant because in the end our prediction and hypothesis was supported. I thought that this was an interesting lab to participate in especially because this experiment has been conducted several times by other biology labs, I get intot really believe there were any significant weaknesses to our experiment except maybe the time. It would have been better to have more time to further explore our results. This experiment was conducted smoothly and without complications, and even better supported our prediction. Some questions that would be interesting to be answered by further look into is would temperature affect the rate of diffusion in sucrose concentrations?Works Cite dFreeman, Scott. Lipids, Membranes, and the First Cells. Biological Sciences. 4th ed. Vol. 1. Boston McGraw Hill, 2011. 90-91. Print. Karp, Gerald. Cell and Molecular Biology Concepts and Experiments. spic-and-span York J. Wiley, 1999. Print Marvel, Stephen C., and Megan V. Kepler. A Simple Membrane Osmometer System & Experiments That quantitatively Measure Osmotic Pressure. The American Biology Teacher 6.7 (2009) 355-62. Print. Mccandless, John. BIOLOGY.ARIZONA.EDU. BIOLOGY.ARIZONA.EDU. University of Arizona, 27 Feb. 1997. Web. 26 Feb. 2012. .